不同水平的亚硒酸钠对鸡心肌组织硒沉积及组织结构的影响

通过在公鸡日粮中添加不同水平的亚硒酸钠,研究硒在公鸡心肌组织中的沉积量和对其组织结构功能的影响,以期为亚硒酸钠在禽类生产中的应用提供参考依据。试验选取80只体重接近、健康无疾病的海兰白成年公鸡,随机分成4组,每组20只,分别在日粮中添加0、0.3、0.6和1.0 mg/kg的亚硒酸钠。试验结束时,采集心组织样本,测定硒含量,并通过HE染色观察组织细胞结构。结果表明,公鸡心肌组织中硒含量随日粮硒含量的增加而升高。由此得出,公鸡心肌组织的硒含量随着日粮硒水平的增加而逐渐升高,高硒日粮对心肌组织的结构有一定影响,但由于硒在心肌组织中的沉积能力与其他组织相比较差,因此影响并不明显。     

Influences of the Paddy Rices with Different Moistures on the Head Rice Recovery

A major component rice varietv of Xiangwanxian 9 (a late Indica variety) with 13.1%, 15.6% and 18.3% of the moisture was respectively mixed with three ad-ditive varieties in 13% of moisture at ration of 7:3 for processing assorted rices. Head rice recovery of the treatments was determined 24 hours later. Analysis indicated sig-nificant differences exist in the effects produced by the component varieties, their moistures and the component variety moisture.     

黄金梨和鸭梨叶片光合作用的光抑制及其恢复的比较研究

 以黄金梨和鸭梨的连体和离体叶片为研究材料, 在自然光强和模拟光强下测定光合作用和叶绿素荧光参数。结果表明: 黄金梨和鸭梨连体叶片经午间强光处理后, 净光合速率( P_n) 、表观量子效率(AQY) 和光系统Ⅱ ( PSⅡ) 的光化学效率(F_v /F_m ) 降低, 初始荧光( F_o ) 升高, 且其降低或升高的幅度随着光强的增强而加大, 说明强光胁迫使叶片发生了光抑制; 黄金梨和鸭梨离体叶片的光抑制程度均随着强光照射时间的延长而加重, 但是在光强减弱或在弱光下恢复2～4 h, 均可基本恢复到正常值, 说明仅强光引起的光抑制是可逆的; 对DTT处理的叶片进行强光处理, 其F_v/F_m比未吸入DTT的叶片低, F_o比对照高, 说明依靠叶黄素循环进行热耗散是梨树叶片防御强光破坏光合机构的重要途径之一; 黄金梨对强光胁迫的忍耐能力和恢复能力较鸭梨弱, 说明梨树光抑制程度品种间有差异。     

蛋氨酸硒对肉用仙居鸡生长性能、硒存留率和胴体特性的影响

采用饲养试验、代谢试验、屠宰测定等方法 ,研究在基础日粮中添加不同剂量蛋氨酸硒对肉用仙居鸡生长性能和胴体特性的影响。结果表明 ,在基础日粮中分别添加 0 .1,0 .15 ,0 .2和 0 .2 5mg/kgSe水平的蛋氨酸硒比对照组 (添加 0 .15mg/kgSe水平的亚硒酸钠 )体增重分别增加了 9.5 4 % ,12 .30 % ,9.0 7%和 8.0 4 % (P <0 .0 5 ) ;饲料转化率分别提高了 7.35 % ,9.4 5 % ,9.19%和 8.4 0 % (P <0 .0 5 ) ;硒存留率分别提高 2 9.92 % ,2 6 .4 2 % ,19.88%和 19.6 4 %(P <0 .0 5 ) ;蛋氨酸硒对鸡的胴体特性无显著影响     

Yield, water and nitrogen-use response of rice to zeolite and nitrogen fertilization in a semi-arid environment

Water scarcity and soil nitrogen (N) loss are important limitations for agricultural production in semi-arid region especially for rice production. Zeolite (Z) as a soil conditioner can be used to retrain water and nitrogen in near-surface soil layer in lowland rice production system. The objectives of this study were to investigate the effects of different application rates of natural zeolite (clinoptilolite) and nitrogen on rice yield, yield components, soil nitrogen, water use, water productivity in a silty clay soil in 2004 and 2005. Zeolite was only applied in the first year. In order to study the long-term and continuous effect of zeolite on the objectives of the study, no zeolite was applied in the second year and the study was conducted on the same land as the first year. Zeolite and N were applied at rates of 0, 2, 4, and 8 t ha⁻¹ and 0, 20, 40, and 80 kg ha⁻¹, respectively in 2004. In 2005, each plot received the same amount of N as received in 2004. It is concluded that by decreasing N application rates, higher Z application rate is needed to improve grain yield. Highest grain yield was obtained at N application rate of 80 kg ha⁻¹ and Z application rate of 4 t ha⁻¹. Higher grain yield was mostly attributed to lower unfilled grain percentage and higher 1000-grain weight that were a result of higher N application rate and N retention in soil due to Z application. Nitrogen and Z applications resulted in higher grain protein contents and nitrogen recovery efficiency (NRE). Based on these results and due to higher N retention in soil under Z application, improved grain yield quality, nitrogen-use efficiency (NUE), and nitrogen recovery efficiency (NRE) could be obtained at Z application rate of 8 t ha⁻¹ and N application rate of 80 kg ha⁻¹ or more. However, this was not satisfied for NUE. Moreover, it is found that at higher N application rates lower Z application rates are needed to effectively retain soil residual mineral nitrogen. Furthermore, at N application rates of 80 kg ha⁻¹ or more, Z application increased soil water retention and resulted in lower seasonal water use and higher water productivity. In general, it was concluded that the effect of Z application in retaining soil N was also effective in the second year.     

不同烧伤程度森林利用与恢复的观测研究

按照火灾后林木烧伤等级的不同,分幼龄林、中龄林、近成过3个龄级组按阳坡、阴坡、平坡以及沟塘4种类型分别设标准地进行观测研究,为火灾后森林是否采留提供依据,并实现森林利用价值的最大化。     

西藏“一江两河”中部流域河谷沙化草地及其恢复途径探讨

“一江两河”中部流域是西藏沙漠化最为严重的地区之一，草地的沙化对于生态环境的恶化和畜牧业生产造成很大的不利影响。通过对这一地区草地的现状分析，划分了沙化草地类型，并通过示范试验结果分析，探讨了沙化草地恢复的主要措施。     

梅花品种抗寒性的比较分析

用电导法和生长法测定了梅花38个主要代表品种的抗寒性,并对梅花的系统、类、型的抗寒性进行了比较。结果表明,杏梅系统比真梅系统抗寒,真梅系统的直脚梅类和垂枝梅类比龙游梅类抗寒。在直脚梅类的六个型中,玉蝶型和绿萼型抗寒性较强,朱砂型抗寒弱。垂枝梅类的‘骨红垂枝’同直脚梅类的朱砂型抗寒性相似。对梅花品种抗寒性的季节性变化、同型及同品种在不同栽培地区的差异及其枝条脱水等也进行了一些讨论。提出了在北京地区引种栽培梅花的几点意见。     

DNA recovery from agarose gels with a simple centrifuge-driven sephadex filtration

Conventional methods of DNA recovery from agarose gel generally require expensive equipment, extended elution times, or considerable handling of the sample after elution. We developed a simple protocol for a quick and effective recovery of DNA from agarose gels with good yield and quality. Using a Sephadex resin filled spin column, DNA fragments of 500 bp to 6 kb in an agarose gel slice were easily recovered by a 2 min centrifugation. The recovery efficiencies were over 40%-50% and the eluted DNA can be used directly for downstream application, such as polymerase chain reactions (PCR) and restriction enzyme digestion. This method could also be used to recover large DNA fragment (48 kb) without degradation. The use of Sephadex helps to remove small molecular impurities from agarose and it also reduces the chance of clogging the column filter caused by direct contact with agarose.     

Effects of detomidine or romifidine during maintenance and recovery from isoflurane anaesthesia in horses

ObjectiveTo evaluate the effects of detomidine or romifidine on cardiovascular function, isoflurane requirements and recovery quality in horses undergoing isoflurane anaesthesia.Study designProspective, randomized, blinded, clinical study.AnimalsA total of 63 healthy horses undergoing elective surgery during general anaesthesia.MethodsHorses were randomly allocated to three groups of 21 animals each. In group R, horses were given romifidine intravenously (IV) for premedication (80 μg kg^–1), maintenance (40 μg kg^–1 hour^–1) and before recovery (20 μg kg^–1). In group D2.5, horses were given detomidine IV for premedication (15 μg kg^–1), maintenance (5 μg kg^–1 hour^–1) and before recovery (2.5 μg kg^–1). In group D5, horses were given the same doses of detomidine IV for premedication and maintenance but 5 μg kg^–1 prior to recovery. Premedication was combined with morphine IV (0.1 mg kg^–1) in all groups. Cardiovascular and blood gas variables, expired fraction of isoflurane (Fe′Iso), dobutamine or ketamine requirements, recovery times, recovery events scores (from sternal to standing position) and visual analogue scale (VAS) were compared between groups using either anova followed by Tukey, Kruskal-Wallis followed by Bonferroni or chi-square tests, as appropriate (p < 0.05).ResultsNo significant differences were observed between groups for Fe′Iso, dobutamine or ketamine requirements and recovery times. Cardiovascular and blood gas measurements remained within physiological ranges for all groups. Group D5 horses had significantly worse scores for balance and coordination (p = 0.002), overall impression (p = 0.021) and final score (p = 0.008) than group R horses and significantly worse mean scores for VAS than the other groups (p = 0.002).Conclusions and clinical relevanceDetomidine or romifidine constant rate infusion provided similar conditions for maintenance of anaesthesia. Higher doses of detomidine at the end of anaesthesia might decrease the recovery quality.